describe Pneumococcal vaccine in complement deficiency mice.

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[ My subject is Infection and Immunity]

Tittle: Pneumococcal vaccine in complement deficiency mice.

1- I need it very high quality PhD level in immunology with proofreading and editing ( UK writer style ).
2- (( I need you to provide my Refworks references file for the references which you used )).
3- don’t repeat the information between the subheading.

Introduction:

C1q Protein in mice:
I wrote about The Classical Pathway of Complement Activation, but I need more specific information about C1q protein with its structure and put figure about it. (200 words).

C1q Deficiency in mice (C1q-/-):
I wrote about Deficiencies of the classical pathway, but I need more specific information about C1q Deficiency in mice. (200 words).

MASP-2 Protein in mice:
I wrote about Lectin Pathway of Complement Activation, but I need more specific information about MASP-2 Protein with its structure and put figure about it. (200 words).

MASP-2 Deficiency in mice (MASP-2-/-):
I wrote about Deficiencies of the Lectin pathway, but I need more specific information about MASP-2 Deficiency in mice. (200 words).

Factor B Protein in mice (fB):
I wrote about The alternative Pathway of Complement Activation, but I need more specific information about factor B protein with its structure and put figure about it. (200 words).

Factor B Deficiency in mice (fB-/-):
I wrote about Deficiencies of the alternative pathway, but I need more specific information about factor B Deficiency in mice. (200 words).

Antibody structure in mice:
General information with structures’ figures (212 words).

Then more details about IgG antibody in mice (150 words).

Produced IgG Antibody in mice immunised with Pneumococcal Polysaccharide Vaccine (PneumovaxII): (200 words)

Produced IgG Antibody in mice immunised with Pneumococcal Polysaccharide Conjugate Vaccine (Prevnar 13): (200 words)

Produced IgG Antibody in mice immunised with CRM197 Protein ( non-toxic mutant of diphtheria toxin): (200 words)

Anti-pneumococcal antibody in mice:
General information (200 words).

Anti-pneumococcal polysaccharide vaccines antibody:
General information (200 words).

Then add more details about the vaccines components (Anti-CRM197 protein antibody in mice, Anti-pneumococcal polysaccharide vaccine (PneumovaxII) antibody and Anti-prevenar 13 vaccine antibody in mice). My study focusing on IgG level. Mice immunised with 20 μg of CRM197 protein single or multiple immunisation. Another group of mice immunised with 1 μg of PneumovaxII vaccine single or multiple immunisation. Final group of mice immunised with Prevenar13 (which is contain 13 types of Pneumococcal polysaccharide and CRM197 protein) the mice dose is (human dose /25 which equal same velum 1 μg of PneumovaxII vaccine) single or multiple immunisation. (313 words)

Antibody titre in mice:
General information (200 words).

Measurement of IgG antibody level using ELISA technique (200 words).

B and T cells responses in mice (This title you can divide it to 2 titles):
General information (200 words).
Focusing on CD’s antibody markers (( CD3, CD4, CD8, CD25, CD19 and CD45R/B220 )) which I used in my study to measure B and T cells responses in spleen cells in mice immunised with CRM197 or PneumovaxII or Prevenar13 .

Total about ((( 3575 words ))) 13 pages

(( NOTIES: Please, don’t rebate the information between the subtitles)))
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